Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Light-induced Ca(2+) transients observed in widefield epi-fluorescence microscopy of excitable cells

McDonald, Alison and Harris, John and MacMillan, Debbi and Dempster, John and McConnell, Gail (2012) Light-induced Ca(2+) transients observed in widefield epi-fluorescence microscopy of excitable cells. Biomedical Optics Express, 3 (6). pp. 1266-1273. ISSN 21567085

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

We have investigated the possibility that variations in the level of intracellular Ca(2+) in excitable cells might be induced as an artifact of the incoherent illumination that is being used to monitor transient responses. In order to avoid the fluctuations in power of an arc lamp source, a microscope using a light emitting diode that was calibrated accurately at low power levels, was constructed to provide good control over the dose of light applied to the biological specimen. We report here that higher powers of illumination increased the probability of occurrence of Ca(2+) transients even in the sub-mW range normally used to measure such transients in epi-fluorescence work, suggesting that caution should be exercised when designing experiments and interpreting data

Item type: Article
ID code: 40250
Keywords: epi-fluorescence microscopy , excitable cells, biomedical optics, Therapeutics. Pharmacology, Biotechnology, Atomic and Molecular Physics, and Optics
Subjects: Medicine > Therapeutics. Pharmacology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
Depositing user: Pure Administrator
Date Deposited: 29 Jun 2012 10:25
Last modified: 27 Mar 2014 10:13
URI: http://strathprints.strath.ac.uk/id/eprint/40250

Actions (login required)

View Item