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Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I

Thanakiatkrai, Phuvadol and Welch, Lindsey (2012) Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I. International Journal of Legal Medicine, 126 (1). pp. 161-165. ISSN 0937-9827

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Abstract

Here, we applied the Taguchi method, an engineering optimization process, to successfully determine the optimal conditions for three SYBR Green I-based quantitative PCR assays. This method balanced the effects of all factors and their associated levels by using an orthogonal array rather than a factorial array. Instead of running 27 experiments with the conventional factorial method, the Taguchi method achieved the same optimal conditions using only nine experiments, saving valuable resources.