Thanakiatkrai, Phuvadol and Welch, Lindsey (2012) Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I. International Journal of Legal Medicine, 126 (1). pp. 161-165. ISSN 0937-9827Full text not available in this repository. (Request a copy from the Strathclyde author)
Here, we applied the Taguchi method, an engineering optimization process, to successfully determine the optimal conditions for three SYBR Green I-based quantitative PCR assays. This method balanced the effects of all factors and their associated levels by using an orthogonal array rather than a factorial array. Instead of running 27 experiments with the conventional factorial method, the Taguchi method achieved the same optimal conditions using only nine experiments, saving valuable resources.
|Keywords:||real-time PCR, SYBR Green I, factorial, qPCR, DNA amplification, Taguchi, optimization, orthogonal, Chemistry, Pathology and Forensic Medicine|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry|
|Depositing user:||Pure Administrator|
|Date Deposited:||22 Jun 2012 15:36|
|Last modified:||07 Jan 2017 01:13|