Thanakiatkrai, Phuvadol and Welch, Lindsey (2012) Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I. International Journal of Legal Medicine, 126 (1). pp. 161-165. ISSN 0937-9827
Full text not available in this repository. (Request a copy from the Strathclyde author)Official URL: http://dx.doi.org/10.1007/s00414-011-0558-5
Abstract
Here, we applied the Taguchi method, an engineering optimization process, to successfully determine the optimal conditions for three SYBR Green I-based quantitative PCR assays. This method balanced the effects of all factors and their associated levels by using an orthogonal array rather than a factorial array. Instead of running 27 experiments with the conventional factorial method, the Taguchi method achieved the same optimal conditions using only nine experiments, saving valuable resources.
| Item type: | Article |
|---|---|
| ID code: | 40204 |
| Keywords: | real-time PCR, SYBR Green I, factorial, qPCR, DNA amplification, Taguchi, optimization, orthogonal, Chemistry |
| Subjects: | Science > Chemistry |
| Department: | Faculty of Science > Pure and Applied Chemistry |
| Related URLs: | |
| Depositing user: | Pure Administrator |
| Date Deposited: | 22 Jun 2012 16:36 |
| Last modified: | 22 Jun 2012 16:36 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/40204 |
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