Picture of two heads

Open Access research that challenges the mind...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

Discover more...

Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I

Thanakiatkrai, Phuvadol and Welch, Lindsey (2012) Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I. International Journal of Legal Medicine, 126 (1). pp. 161-165. ISSN 0937-9827

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Here, we applied the Taguchi method, an engineering optimization process, to successfully determine the optimal conditions for three SYBR Green I-based quantitative PCR assays. This method balanced the effects of all factors and their associated levels by using an orthogonal array rather than a factorial array. Instead of running 27 experiments with the conventional factorial method, the Taguchi method achieved the same optimal conditions using only nine experiments, saving valuable resources.