Picture of wind turbine against blue sky

Open Access research with a real impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

The Energy Systems Research Unit (ESRU) within Strathclyde's Department of Mechanical and Aerospace Engineering is producing Open Access research that can help society deploy and optimise renewable energy systems, such as wind turbine technology.

Explore wind turbine research in Strathprints

Explore all of Strathclyde's Open Access research content

Development and validation of a fluorescence method to follow the build-up of short peptide sequences on solid 2D surfaces

Zelzer, Mischa and Scurr, David J. and Alexander, Morgan R. and Ulijn, Rein V. (2012) Development and validation of a fluorescence method to follow the build-up of short peptide sequences on solid 2D surfaces. ACS Applied Materials and Interfaces, 4 (1). pp. 53-58. ISSN 1944-8244

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

The modification of material surfaces with short peptide sequences has become an essential step in many biotechnological and biomedical applications. Due to their simple architecture compared to more complex 3D substrates, 2D surfaces are of particular interest for high throughput applications and as model surfaces for dynamic or responsive surface modifications. The decoration of these surfaces with peptides is commonly accomplished by synthesizing the peptide first and subsequently transferring it onto the surface of the substrate. Recently, several procedures have been described for the synthesis of peptides directly onto a 2D surface, thereby simplifying and accelerating the modification of flat surfaces with peptides. However, the wider use of these techniques requires a routine method to monitor the amino acid build-up on the surface. Here, we describe a fast, inexpensive and nondestructive fluorescence based method which is readily accessible to follow the amino acid build-up on solid 2D samples.