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Inactivating alternative NADH dehydrogenases : enhancing fungal bioprocesses by improving growth and biomass yield?

Voulgaris, Ioannis and O'Donnell, Andrew and Harvey, Linda and McNeil, Brian (2012) Inactivating alternative NADH dehydrogenases : enhancing fungal bioprocesses by improving growth and biomass yield? Scientific Reports, 2. -. ISSN 2045-2322

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Abstract

Debate still surrounds the physiological roles of the alternative respiratory enzymes found in many fungi and plants. It has been proposed that alternative NADH:ubiquinone oxidoreductases (NADH dehydrogenases) may protect against oxidative stress, conversely, elevated activity of these enzymes has been linked to senescence. Here we show that inhibition of these enzymes in a fungal protein expression system (Aspergillus niger) leads to significantly enhanced specific growth rate, substrate uptake, carbon dioxide evolution, higher protein content, and more efficient use of substrates. These findings are consistent with a protective role of the NADH dehydrogenases against oxidative stress, thus, when electron flow via these enzymes is blocked, flux through the main respiratory pathway rises, leading to enhanced ATP generation. We anticipate that our findings will stimulate further studies in fungal and plant cultures leading to significant improvements in these expression systems, and to deeper insights into the cellular roles of alternative respiration.

Item type: Article
ID code: 39938
Keywords: adenosine triphosphate, biomass, carbon dioxide, fungi, glucose, NADH dehydrogenases , quaternary ammonium compounds, superoxides, Pharmacy and materia medica, General
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 06 Jun 2012 14:18
    Last modified: 01 Oct 2014 14:54
    URI: http://strathprints.strath.ac.uk/id/eprint/39938

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