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Loss of Gli3 enhances the viability of embryonic telencephalic cells in vitro

Zaki, Paulett A and Martynoga, Ben and Delafield-Butt, J T and Fotaki, V and Yu, T and Price, D J (2005) Loss of Gli3 enhances the viability of embryonic telencephalic cells in vitro. European Journal of Neuroscience, 22 (6). pp. 1547-1551. ISSN 0953-816X

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Abstract

The transcription factor Gli3 is important for brain and limb development. Mice homozygous for a mutation in Gli3 (Gli3(Xt/Xt)) have severe abnormalities of telencephalic development and previous studies have suggested that aberrant cell death may contribute to the Gli3(Xt/Xt) phenotype. We demonstrate that telencephalic cells from embryonic Gli3(Xt/Xt) embryos survive better and are more resistant to death induced by cytosine arabinoside, a nucleoside analogue that induces death in neuronal progenitors and neurons, than are control counterparts in vitro. Culture medium conditioned by Gli3(Xt/Xt) cells is more effective at enhancing the viability of control telencephalic cells than medium conditioned by control cells, indicating that Gli3(Xt/Xt) cells release a factor or factors which enhance telencephalic cell viability. Gli3(Xt/Xt) cells are also more sensitive to released factors present in conditioned media. These data suggest that Gli3 plays both cell-autonomous and cell-nonautonomous roles in mediating telencephalic cell viability.

Item type: Article
ID code: 39811
Keywords: cell death, mouse, primary culture, Neuroscience. Biological psychiatry. Neuropsychiatry, Neuroscience(all)
Subjects: Medicine > Internal medicine > Neuroscience. Biological psychiatry. Neuropsychiatry
Department: Faculty of Humanities and Social Sciences (HaSS) > School of Education > Education
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 29 May 2012 10:32
    Last modified: 05 Sep 2014 16:06
    URI: http://strathprints.strath.ac.uk/id/eprint/39811

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