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Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleoptile protoplasts

Thiel, G and Sutter, J U and Homann, U (2000) Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleoptile protoplasts. Pflugers Archiv - European Journal of Physiology, 439 (3 Supp). R152-3. ISSN 0031-6768

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Abstract

Ca2+ and osmotic driven extension of the surface area of maize coleoptile protoplasts was investigated using capacitance measurements and photolysis of the caged compound DM-nitrophen. Protoplasts responded to an elevation of cytoplasmic Ca2+ (Ca(i)) with a rapid burst in capacitance reaching a maximal increase of 1.3+/-1.1% over the resting cell capacitance. Subsequent lowering of the osmotic potential in the external medium by 210 mosmol caused a further increase in Cm by 26+/-6%. These data indicate two independent pathways for insertion of membrane into the plasma membrane. One is driven by Ca(i) and recruits membrane from a small pool. The osmotic evoked rise in surface area draws membrane from a much larger reservoir and may be driven by membrane tension.