Picture of scraped petri dish

Scrape below the surface of Strathprints...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Explore world class Open Access research by researchers at Strathclyde, a leading technological university.

Explore

CdSe/ZnS core/shell quantum dots as luminescence lifetime sensors for Cu2+

Sutter, Jens and Birch, David and Rolinski, Olaf J. (2012) CdSe/ZnS core/shell quantum dots as luminescence lifetime sensors for Cu2+. Measurement Science and Technology, 23 (5). ISSN 0957-0233

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

We report the use of CdSe/ZnS core/shell quantum dots QDot800 (Invitrogen) as luminescence lifetime sensors for copper ions Cu2+(H2O)8 in solution with a sensitivity of <1 ppb that is relevant to intracellular copper concentrations. Excitation of QDot800 at 485 nm was found to be optimum in that it did not cause any change in the level of luminescence intensity or luminescence lifetime in the absence of copper ions. When excited at 485 nm a bi-exponential luminescence decay of QDot800 was observed suggesting the presence of two distinct emitting states, both capable of undergoing metal ion quenching that facilitates Cu2+ detection. Selectivity for copper, as against other transition metal ions, as well as other evidence, suggests the primary origin of the quenching is luminescence resonance energy transfer to both free and bound copper ions. The luminescence kinetics of quantum dots and their optimization and applicability for resonance energy transfer-based lifetime sensing in general is discussed.