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Phospholipase C mediates cadmium-dependent apoptosis in HEK 293 cells

Lawal, Akeem O and Ellis, Elizabeth M (2012) Phospholipase C mediates cadmium-dependent apoptosis in HEK 293 cells. Basic and Clinical Pharmacology and Toxicology, 110 (6). pp. 510-517. ISSN 1742-7843

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Abstract

Cadmium is a heavy metal that is known to cause toxicity to cells and, at low concentrations, can initiate apoptosis. This study was undertaken with the aim of defining the role of phospholipase C (PLC) in mediating cadmium-induced apoptosis in human embryonic kidney (HEK 293) cells. We have shown that intracellular Ca(2+) levels increased significantly in HEK 293 cells after 24-hr exposure to Cd. The activity of the calcium-dependent protease calpain rose by four times. The PLC-specific inhibitor, U73122, prevented the Cd-dependent increase in Ca(2+) levels and also abolished Cd-dependent calpain and caspase 3 activation as well as Cd-dependent mitochondrial Bax accumulation. Inhibition of PLC also leads to an increased cell viability following exposure to Cd. Taken together, the results show that the PLC pathway is involved in mediating Cd-induced apoptosis in HEK 293 cells.

Item type: Article
ID code: 39161
Notes: © 2012 The Authors. Basic & Clinical Pharmacology & Toxicology © 2012 Nordic Pharmacological Society.
Keywords: phospholipase C, apoptosis , Therapeutics. Pharmacology, Toxicology, Pharmacology
Subjects: Medicine > Therapeutics. Pharmacology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
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Depositing user: Pure Administrator
Date Deposited: 16 Apr 2012 10:16
Last modified: 27 Mar 2014 10:03
URI: http://strathprints.strath.ac.uk/id/eprint/39161

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