Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Protein kinase involved in flagellar-length control

Wiese, M. and Kuhn, D. and Grunfelder, C.G. (2003) Protein kinase involved in flagellar-length control. Eukaryotic Cell, 2 (4). pp. 769-777. ISSN 1535-9778

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

During its life cycle, the parasitic protozoon Leishmania mexicana differentiates from a flagellated form, the promastigote, to an amastigote form carrying a rudimentary flagellum. Besides biochemical changes, this process involves a change in overall cell morphology including flagellar shortening. A mitogen-activated protein kinase kinase homologue designated LmxMKK was identified in a homology screening and found to be critically involved in the regulation of flagellar assembly and cell size. LmxMKK is exclusively expressed in the promastigote stage and is likely to be regulated by posttranslational mechanisms such as phosphorylation. A deletion mutant for the single-copy gene revealed motile flagella dramatically reduced in length and lacking the paraflagellar rod, a structure adjacent to the axoneme in kinetoplastid flagella. Moreover, a fraction of the cells showed perturbance of the axonemal structure. Complementation of the deletion mutant with the wild-type gene restored typical promastigote morphology. We propose that LmxMKK influences anterograde intraflagellar transport to maintain flagellar length in Leishmania promastigotes; as such, it is the first protein kinase known to be involved in organellar assembly.

Item type: Article
ID code: 39076
Keywords: protein kinase, flagellar-length control, eukaryotic cells, Pharmacy and materia medica, Molecular Biology, Microbiology
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 11 Apr 2012 11:54
    Last modified: 04 Sep 2014 19:25
    URI: http://strathprints.strath.ac.uk/id/eprint/39076

    Actions (login required)

    View Item