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The effect of hyposmotic and isosmotic cell swelling on the intracellular [Ca-2+] in lactating rat mammary ACinar cells

Shennan, D. and Grant, A.C.G. and Gow, I.F. (2002) The effect of hyposmotic and isosmotic cell swelling on the intracellular [Ca-2+] in lactating rat mammary ACinar cells. Molecular and Cellular Biochemistry, 233 (1-2). pp. 91-97. ISSN 0300-8177

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Abstract

The effect of hyposmotic and isosmotic cell swelling on the free intracellular calcium concentration ([Ca2+]i) in rat mammary acinar cells has been examined using the fura-2 dye technique. A hyposmotic shock (40% reduction) increased the [Ca2+]i in rat mammary acinar cells in a fashion which was transient; the [Ca2+]i returned to a value similar to that found under isomotic conditions within 180 sec. The increase in the [Ca2+]i was dependent upon the extent of the osmotic shock. The hyposmotically-activated increase in the [Ca2+]i could not be attributed to a reduction in extracellular Na+ or a change in the ionic strength of the incubation medium. Thapsigargin (1 M) enhanced the hyposmotically-activated increase in the [Ca2+]i. Isosmotic swelling of rat mammary acinar cells, using urea, had no significant effect on the [Ca2+]i. Similarly, a hyperosmotic shock did not affect the [Ca2+]i in rat mammary acinar cells. It appears that the effect of cell swelling on the [Ca2+]i in rat mammary acinar cells depends on how the cells are swollen (hyposmotic vs. isosmotic). This finding may have important physiological implications given that it is predicted that mammary cell volume will change in vivo under isomotic conditions.

Item type: Article
ID code: 38727
Keywords: mammary, calcium , cell swelling, Pharmacy and materia medica, Cell Biology, Molecular Biology, Clinical Biochemistry
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 27 Mar 2012 12:23
    Last modified: 04 Sep 2014 19:11
    URI: http://strathprints.strath.ac.uk/id/eprint/38727

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