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Metabolism of quercetin and kaempferol by rat hepatocytes and identification of flavonoid glycosides in plasma

Oliveira, E.D.J. and Watson, D.G. and Grant, M.H. (2002) Metabolism of quercetin and kaempferol by rat hepatocytes and identification of flavonoid glycosides in plasma. Xenobiotica, 32 (4). pp. 279-287. ISSN 0049-8254

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Abstract

The metabolism of the flavonoids quercetin and kaempferol by rat hepatocytes was investigated using liquid chromatography coupled with electrospray mass spectrometry (LC-ESI MS). Quercetin and kaempferol were extensively metabolized (98.8 +/- 0.1% and 81.0 +/- 5.1% respectively, n = 4), with four glucuronides of quercetin and two of kaempferol being detected after incubation. 2. The glucuronides of quercetin and kaempferol formed upon incubation with rat hepatocytes were identified as the same ones formed after incubation with the UDP-glucuronosyltransferase isoform UGT1A9. 3. In addition, plasma samples from human volunteers taken after consumption of capsules of Ginkgo biloba, a plant rich in flavonoid glycosides, were analysed by LC-MS for the presence of flavonoid glucuronides and flavonoid glycosides. Reported is evidence for the presence of flavonoid glycosides in samples of plasma. 4. The results suggest that UGT1A9 is a key UDP-glucuronosyltransferase isoform for the metabolism of flavonoids, and that absorption of intact flavonoid glycosides is possible.

Item type: Article
ID code: 38580
Keywords: quercetin , kaempferol , rat hepatocytes , plasma, flavonoid glycosides, Therapeutics. Pharmacology, Bioengineering, Medicine(all)
Subjects: Medicine > Therapeutics. Pharmacology
Technology > Engineering (General). Civil engineering (General) > Bioengineering
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences > Pharmaceutical Sciences
Faculty of Engineering > Bioengineering
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    Depositing user: Pure Administrator
    Date Deposited: 20 Mar 2012 11:45
    Last modified: 04 Sep 2014 19:10
    URI: http://strathprints.strath.ac.uk/id/eprint/38580

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