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Examining structure-activity correlations of some high activity enzyme preparations for low water media

Solanki, Kusum and Halling, Peter J. and Gupta, Munishwar N. (2012) Examining structure-activity correlations of some high activity enzyme preparations for low water media. Bioresource Technology, 115 (July). pp. 147-151.

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Abstract

A first study of the comparison of structures of enzymes (by FT-IR and CD) in different high activity (in low water media) preparations is reported. Using chymotrypsin and subtilisin as models, we have studied various factors that distinguish enzyme precipitated and rinsed with propanol (EPRP), crosslinked enzyme aggregates (CLEA), protein coated microcrystals (PCMC) and crosslinked protein coated microcrystals (CLPCMC). The suspensions in organic media were assayed for catalytic activity, and structures were probed by FT-IR and CD measurements. CD studies of enzyme suspensions were possible by using a rotating cell accessory. There was a generally good correlation between higher catalytic activity and retention of native structures. Activity and retention of native structure was always higher if aqueous enzyme solution was added to propanol rather than vice versa in the precipitation step of these preparations. The work identifies factors which may lead to better biocatalyst designs for low water media.

Item type: Article
ID code: 38052
Notes: Examining structure-activity correlations of some high activity enzyme preparations for low water media
Keywords: alpha-chymotrypsin, subtilisin, FT-IR, enzymes , low water media, Chemistry, Chemistry (miscellaneous)
Subjects: Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
Depositing user: Pure Administrator
Date Deposited: 01 Mar 2012 12:12
Last modified: 27 Mar 2014 10:00
URI: http://strathprints.strath.ac.uk/id/eprint/38052

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