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Pharmaceutical development of the novel arsenical based cancer therapeutic GSAO for phase I clinical trial

Elliott, Moira and Ford, S J and Prasad, E and Dick, L J and Farmer, H and Hogg, P J and Halbert, G W (2012) Pharmaceutical development of the novel arsenical based cancer therapeutic GSAO for phase I clinical trial. International Journal of Pharmaceutics, 426 (1-2). pp. 67-75.

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Abstract

The novel organoarsenical GSAO, 4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid, has potential anti-angiogenic capability with application in cancer where tumour metastasis relies on neo-vascularisation. As GSAO arsenic is trivalent, the arsenoxide moiety reacts with appropriately spaced cysteine residues on adenine nucleotide translocase (ANT) mitochondrial membrane protein. Molecular oxidation of the arsenic to the pentavalent structure, as in the degradant GSAA (4-(N-(S-glutathionylacetyl)amino) phenylarsonic acid), prevents sulphydryl interaction and risks abolition of activity. We report here on formulation studies aiming to produce a parenteral product with the primary objective of restricting GSAA transformation from GSAO to protect maximal potency of the molecule. Successful anti-oxidant strategy primarily came from pH control. The presence of glycine was proposed to form a stabilising five-membered oxazarsolidinone ring with arsenoxide and this was investigated using potentiometric assays. We report on these tritration studies identifying a pK(a) of 8.2 associated with an As-OH, but not confirming ring presence. An original clinical trial pharmaceutical was successfully realised by lyophilisation of 50mg/mL GSAO in 100mM glycine solution, pH 7 to obtain a 48-month shelf life for the freeze-dried vials. The Phase I clinical study is ongoing in patients with solid tumours refractory to standard therapy.

Item type: Article
ID code: 37914
Notes: Copyright © 2012 Elsevier B.V. All rights reserved.
Keywords: phenylarsonous acid , cancer, lyophilisation , tumours, Therapeutics. Pharmacology, Pharmaceutical Science
Subjects: Medicine > Therapeutics. Pharmacology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
Depositing user: Pure Administrator
Date Deposited: 27 Feb 2012 14:03
Last modified: 27 Mar 2014 09:59
URI: http://strathprints.strath.ac.uk/id/eprint/37914

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