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Volumetric changes to the molecular structure of beta-lactoglobulin processed at high pressure

Vant, Stewart C. and Glen, Norman and Kontopidis, George and Sawyer, Lindsay and Schaschke, Carl J. (2002) Volumetric changes to the molecular structure of beta-lactoglobulin processed at high pressure. High Temperature High Pressure, 34 (6). pp. 705-712. ISSN 0018-1544

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Abstract

We measured in-situ the volumetric changes to the molecular structure of the bovine milk protein β-lactoglobulin in a series of high-pressure processing experiments. We used the so-called μPVT device at hydrostatic pressures up to 500 MPa under isothermal conditions of 25 °C, the protein in solution, prepared at both pH 5.0 and 7.0, in a series of compression and decompression cycles. Significant irreversible volumetric changes were found, most notably with a marked step decrease in molar volume of 1.3 litre mol-1 occurring between 10 and 17 MPa. Irreversible molar volume changes of 2.16 litre mol-1 were found when pressures above 17 MPa were applied. This is thought to be due in part to a collapse of the inner calyx. Volume changes were confirmed by measuring the unit-cell volume of the crystalline protein. The effects of pH were indistinguishable. These pressures are considerably less than previously thought necessary for this protein.

Item type: Article
ID code: 3789
Keywords: volumetric changes, molecular structure, beta-lactoglobulin, high pressure, high temperature, Chemical technology, Materials Science (miscellaneous), Mechanics of Materials, Physical and Theoretical Chemistry, Computational Mechanics, Fluid Flow and Transfer Processes
Subjects: Technology > Chemical technology
Department: Faculty of Engineering > Chemical and Process Engineering
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 04 Jul 2007
    Last modified: 04 Sep 2014 11:24
    URI: http://strathprints.strath.ac.uk/id/eprint/3789

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