Zagnoni, Michele (2012) Miniaturised technologies for the development of artificial lipid bilayer systems. Lab on a Chip. ISSN 1473-0197 (In Press)Full text not available in this repository. (Request a copy from the Strathclyde author)
Artificially reproducing cellular environments is a key aim of synthetic biology, which has the potential to greatly enhance our understanding of cellular mechanisms. Microfluidic and lab-on-a-chip (LOC) techniques, which enable the controlled handling of sub-microlitre volumes of fluids in an automated and high-throughput manner, can play a major role in achieving this by offering alternative and powerful methodologies in an on-chip format. Such techniques have been successfully employed over the last twenty years to provide innovative solutions for chemical analysis and cell-, molecular- and synthetic- biology. In the context of the latter, the formation of artificial cell membranes (or artificial lipid bilayers) that incorporate membrane proteins within miniaturised LOC architectures offers huge potential for the development of highly sensitive molecular sensors and drug screening applications. The aim of this review is to give a comprehensive and critical overview of the field of microsystems for creating and exploiting artificial lipid bilayers. Advantages and limitations of three of the most popular approaches, namely suspended, supported and droplet-based lipid bilayers, are discussed. Examples are reported that show how artificial cell membrane microsystems, by combining together biological procedures and engineering techniques, can provide novel methodologies for basic biological and biophysical research and for the development of biotechnology tools.
|Keywords:||lipid bilayer systems , miniaturised technologies, miniaturisation, Electrical engineering. Electronics Nuclear engineering|
|Subjects:||Technology > Electrical engineering. Electronics Nuclear engineering|
|Department:||Faculty of Engineering > Electronic and Electrical Engineering|
?? 119 ??
?? 120 ??
|Depositing user:||Pure Administrator|
|Date Deposited:||13 Feb 2012 15:54|
|Last modified:||12 Jun 2013 14:34|
Actions (login required)