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The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

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Measurement of ionised magnesium in HEPES-buffered serum samples

Gow, Iain F. (2001) Measurement of ionised magnesium in HEPES-buffered serum samples. Analytica Chimica Acta, 432 (1). pp. 143-149. ISSN 0003-2670

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Abstract

The objective of the research is to develop a method of adjusting the pH of serum samples to 7.4, thus allowing measurement of ionised magnesium (Mg2+) at physiological pH without the need to maintain samples under anaerobic conditions. Levels of Mg2+ HEPES-buffered samples were compared with results obtained when serum samples were buffered with CO2, and the Mg2+ level at pH 7.4 calculated from the log form of the Siggaard-Andersen equation. The HEPES buffering technique was also used to measure Mg2+ in serum samples from sheep where circulating levels of Mg2+ were elevated by infusion of MgCl2. The mean Mg2+ values obtained when samples (n=6) were buffered with CO2 or 50 or 100 mM HEPES were not significantly different from each other (mean±S.E.M.; 0.675±0.019, 0.684±0.015, and 0.669±0.015, respectively). When MgCl2 was infused into sheep (n=6), a significant rise in serum Mg2+ was found (mean±S.E.M.; basal 0.613±0.045 mM; peak 1.450±0.095 mM) when using the HEPES-buffering method for sample pre-treatment (final HEPES concentration was 50 mM).