Barber, M.C. and Pooley, L. and Travers, M. (2001) Developmental regulation of alternatively spliced acetyl-co a carboxylase alpha MRNAs encoding isozymes with or without an eight amino acid domain upstream of ser-1200 phosphorylation motif in the mammary gland. Journal of Molecular Endocrinology, 27. pp. 349-356. ISSN 0952-5041Full text not available in this repository. (Request a copy from the Strathclyde author)
Expression of a variant acetyl-CoA carboxylase-alpha (ACC-alpha) mRNA encoding an isozyme either comprising (+24nt) or lacking (Delta24nt) an eight amino acid domain proximal to the Ser-1200 phosphorylation motif has been investigated in ovine and rat mammary tissue throughout pregnancy and lactation. The ratio of the Delta24nt mRNA: +24nt mRNA in ovine tissues varied from 0.1-0.25 (spleen, lung, muscle, heart, adipose tissue, brain) to 0.6-0.8 (pancreas, liver, kidney) to approximately 5.0 (lactating mammary gland). The sixfold increase in total ACC-alpha mRNA expression in mammary gland during lactation was due entirely to a tenfold increase in the level of the Delta24nt species as the level of expression of the +24nt species remained unaltered between pregnancy and lactation. This mode of expression of the +24nt and Delta24nt mRNAs was similar in rat mammary gland. Between day 20 of pregnancy and day 4 of lactation the ratio of the Delta24nt : +24nt mRNA increased from 2:1 to 10-20:1. Forced involution reduced the ratio of the two mRNAs to levels observed throughout pregnancy. Treatment of lactating rats with bromocryptine reduced the ratio of the Delta24nt : +24nt mRNA to relative levels observed after forced involution, suggesting that the exonic splicing responsible for the generation of the two mRNA isoforms is prolactin responsive.
|Keywords:||rat mammary tissue, rat pregnancy, lactation, Pharmacy and materia medica, Molecular Biology, Endocrinology|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Pure Administrator|
|Date Deposited:||03 Feb 2012 15:49|
|Last modified:||06 Jan 2017 06:17|