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G-protein-coupled receptor stimulation of the p42/p44 mitogen-activated protein kinase pathway is attenuated by liPID phosphate phosphatases 1, 1a, and 2 in human embryonic kidney 293 cells

Alderton, Forbes and Darroch, Peter and Sambi, Balwinder and McKie, Amanda and Ahmed, Ikhlas Said and Pyne, Nigel and Pyne, Susan (2001) G-protein-coupled receptor stimulation of the p42/p44 mitogen-activated protein kinase pathway is attenuated by liPID phosphate phosphatases 1, 1a, and 2 in human embryonic kidney 293 cells. Journal of Biological Chemistry, 276. pp. 13452-13460. ISSN 0021-9258

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Abstract

Sphingosine 1-phosphate, lysophosphatidic acid, and phosphatidic acid bind to G-protein-coupled receptors to stimulate intracellular signaling in mammalian cells. Lipid phosphate phosphatases (1, 1a, 2, and 3) are a group of enzymes that catalyze de-phosphorylation of these lipid agonists. It has been proposed that the lipid phosphate phosphatases exhibit ecto activity that may function to limit bioavailability of these lipid agonists at their receptors. In this study, we show that the stimulation of the p42/p44 mitogen-activated protein kinase pathway by sphingosine 1-phosphate, lysophosphatidic acid, and phosphatidic acid, all of which bind to Gi/o-coupled receptors, is substantially reduced in human embyronic kidney 293 cells transfected with lipid phosphate phosphatases 1, 1a, and 2 but not 3. This was correlated with reduced basal intracellular phosphatidic acid and not ecto lipid phosphate phosphatase activity. These findings were supported by results showing that lipid phosphate phosphatases 1, 1a, and 2 also abrogate the stimulation of p42/p44 mitogen-activated protein kinase by thrombin, a peptide Gi/o-coupled receptor agonist whose bioavailability at its receptor is not subject to regulation by the phosphatases. Furthermore, the lipid phosphate phosphatases have no effect on the stimulation of p42/p44 mitogen-activated protein kinase by other agents that do not use G-proteins to signal, such as serum factors and phorbol ester. Therefore, these findings show that the lipid phosphate phosphatases 1, 1a, and 2 may function to perturb G-protein-coupled receptor signaling per se rather than limiting bioavailability of lipid agonists at their respective receptors.

Item type: Article
ID code: 37341
Keywords: kidney cells, receptor stimulation, bioavailability, intracellular signaling, Pharmacy and materia medica, Biochemistry, Cell Biology, Molecular Biology
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences > Physiology and Pharmacology
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 03 Feb 2012 13:33
    Last modified: 04 Sep 2014 18:50
    URI: http://strathprints.strath.ac.uk/id/eprint/37341

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