Karatzaferi, C. and De Haan, A. and Ferguson, R.A. and Van Mechelen, W. and Sargeant, A.J. (2001) Phosphocreatine and ATP content in human single muscle fibres before and after maximum dynamic exercise. Pflugers Archiv - European Journal of Physiology, 442 (3). pp. 467-474. ISSN 0031-6768Full text not available in this repository. (Request a copy from the Strathclyde author)
The recovery of high-energy phosphate levels in single human skeletal muscle fibres following short-term maximal (all-out) exercise was investigated. Three male volunteers exercised maximally for 25 s on an isokinetic cycling ergometer. Muscle biopsy samples from the vastus lateralis were collected at rest, immediately post-exercise and at 1.5 min of recovery. The subjects also performed a second exercise bout 1.5 min after the first, on a separate occasion. Single muscle fibres were dissected, characterized and assigned to one of four groups according to their myosin heavy chain (MyHC) isoform content; namely, type I, IIA, IIAx and IIXa (the latter two groups containing either less or more than 50% IIX MyHC). Fibres were analysed for adenosine 5'-triphosphate (ATP), inosine-5'-monophosphate (IMP), phosphocreatine (PCr) and creatine (Cr) levels. Type I fibres had a lower Cr content than type II fibres (P<0.01). Within type II fibres resting [PCr] increased with increasing MyHC IIX isoform content (r=0.59, P<0.01). Post-exercise [PCr] was very low in all fibre groups (P<0.01 versus rest) while great reductions in ATP were also observed (P<0.01 versus rest), especially in the type II fibre groups. [PCr] at 1.5 min of recovery was still lower compared to rest for all fibre groups (P<0.01) especially in the IIAx and IIXa fibres.
|Keywords:||phosphocreatine, muscle fibres , phosphate , muscle biopsy, exercise, Pharmacy and materia medica, Physiology, Clinical Biochemistry, Physiology (medical)|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Pure Administrator|
|Date Deposited:||31 Jan 2012 15:11|
|Last modified:||29 Apr 2016 08:49|