Mattey, M. (2004) Modelling the mechanisms of glucose transport through cell membrane of aspergillus niger in submerged citric acid fermennation processess. Biochemical Engineering Journal, 20 (1). pp. 7-12. ISSN 1369-703XFull text not available in this repository. (Request a copy from the Strathclyde author)
Data from batch fermentations of citric acid producing Aspergillus niger cultures in shake ﬂasks, loop and stirred tank bioreactors, were used to construct diffusion models for the transport of glucose. It was found that the mediated diffusion model does not reﬂect the relationship between the observed uptake rate and glucose concentration, nor for the lack of sensitivity to citrate. This is due in part of the low value of Km in relation to the actual substrate concentration, which means that the carriers are saturated until the end of the process. The membrane barriers must be strongly inhibited under the standard production conditions. Instead, the simple diffusion model ﬁts all the observed data and it explains the relationship between the speciﬁc uptake rate and the concentration of glucose, which should not exist under carrier-saturated conditions. This may account for the overproduction or organic acids under the speciﬁc process conditions. The simple nature of this mechanism also explains the similarity of the uptake relationships from different sources, despite the use of different growing conditions.
|Keywords:||glucose transport , cell membrane , diffusion models , fed-batch culture, Pharmacy and materia medica, Biomedical Engineering, Bioengineering, Biotechnology, Environmental Engineering|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Pure Administrator|
|Date Deposited:||31 Jan 2012 12:21|
|Last modified:||22 Mar 2017 10:00|