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Modelling the mechanisms of glucose transport through cell membrane of aspergillus niger in submerged citric acid fermennation processess

Mattey, M. (2004) Modelling the mechanisms of glucose transport through cell membrane of aspergillus niger in submerged citric acid fermennation processess. Biochemical Engineering Journal, 20 (1). pp. 7-12. ISSN 1369-703X

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Abstract

Data from batch fermentations of citric acid producing Aspergillus niger cultures in shake flasks, loop and stirred tank bioreactors, were used to construct diffusion models for the transport of glucose. It was found that the mediated diffusion model does not reflect the relationship between the observed uptake rate and glucose concentration, nor for the lack of sensitivity to citrate. This is due in part of the low value of Km in relation to the actual substrate concentration, which means that the carriers are saturated until the end of the process. The membrane barriers must be strongly inhibited under the standard production conditions. Instead, the simple diffusion model fits all the observed data and it explains the relationship between the specific uptake rate and the concentration of glucose, which should not exist under carrier-saturated conditions. This may account for the overproduction or organic acids under the specific process conditions. The simple nature of this mechanism also explains the similarity of the uptake relationships from different sources, despite the use of different growing conditions.

Item type: Article
ID code: 37219
Keywords: glucose transport , cell membrane , diffusion models , fed-batch culture, Pharmacy and materia medica, Biomedical Engineering, Bioengineering, Biotechnology, Environmental Engineering
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 31 Jan 2012 12:21
    Last modified: 04 Sep 2014 19:32
    URI: http://strathprints.strath.ac.uk/id/eprint/37219

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