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The viability and function of primary rat hepatocytes cultured on polymeric membranes developed for hybrid artificial liver devices

Grant, M.H. and Morgan, C. and Henderson, C.J. and Malsch, G. and Seifert, B. and Albrecht, W. and Groth, T. (2005) The viability and function of primary rat hepatocytes cultured on polymeric membranes developed for hybrid artificial liver devices. Journal of Biomedical Materials Research, 73A (3). pp. 367-375. ISSN 0021-9304

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Abstract

Bioartificial liver devices require membranes to support the function and viability of hepatocytes because they are anchorage-dependent cells. This study investigated the ability of several polymeric membranes to support the functions of primary hepatocyte cultures. Tailor-made membranes were sought by synthesizing acrylonitrile copolymers with different comonomers resulting in ionic, hydrophilic, or reactive functional groups on the polymer surface. Hepatocyte morphology and viability were assessed by confocal microscopy, and function by the content and activities of cytochrome P450, and the expression of glutathione S-transferases. Hydrophilic membranes (polyacrylonitrile and acrylonitrile copolymerized with 2-acrylamino-2-methyl-propane sulfonic acid) were more biocompatible than hydrophobic membranes such as polysulfone. The chemistry of the hydrophilic group was important; amine groups had a deleterious effect on maintenance of the primary hepatocytes. The biocompatibility of hydrophobic membranes was improved by collagen coating. Improving the chemistry of membranes for artificial liver devices will enhance the phenotypic stability of the cells, enabling us to prolong treatment times for patients.

Item type: Article
ID code: 3704
Keywords: liver, membranes, bioengineering, biomechanics, medicine, Other systems of medicine, Engineering (General). Civil engineering (General), Physiology, Biology
Subjects: Medicine > Other systems of medicine
Technology > Engineering (General). Civil engineering (General)
Science > Physiology
Science > Natural history > Biology
Department: Faculty of Engineering > Bioengineering
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 05 Jul 2007
    Last modified: 12 Mar 2012 10:39
    URI: http://strathprints.strath.ac.uk/id/eprint/3704

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