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Heterologous protein production using the Pichia pastoris expression system

Macauley-Patrick, Sue and Fazenda, Mariana L. and Mcneil, Brian and Harvey, Linda (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast, 22 (4). pp. 249-270. ISSN 0749-503X

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Abstract

The Pichia pastoris expression system is being used successfully for the production of various recombinant heterologous proteins. Recent developments with respect to the Pichia expression system have had an impact on not only the expression levels that can be achieved, but also the bioactivity of various heterologous proteins. We review here some of these recent developments, as well as strategies for reducing proteolytic degradation of the expressed recombinant protein at cultivation, cellular and protein levels. The problems associated with post-translational modifications performed on recombinant proteins by P. pastoris are discussed, including the effects on bioactivity and function of these proteins, and some engineering strategies for minimizing unwanted glycosylations. We pay particular attention to the importance of optimizing the physicochemical environment for efficient and maximal recombinant protein production in bioreactors and the role of process control in optimizing protein production is reviewed. Finally, future aspects of the use of the P. pastoris expression system are discussed with regard to the production of complex membrane proteins, such as G protein-coupled receptors, and the industrial and clinical importance of these proteins.

Item type: Article
ID code: 36630
Keywords: heterologous expression, yeast , process optimization, post-translational modification, Pichia pastoris, methylotrophic, foreign protein production; , Pharmacy and materia medica, Biochemistry, Applied Microbiology and Biotechnology, Genetics, Bioengineering, Biotechnology
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 11 Jan 2012 14:52
    Last modified: 05 Sep 2014 14:28
    URI: http://strathprints.strath.ac.uk/id/eprint/36630

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