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Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Fast wavelength-tunable ultra-violet laser source for confocal Fura-2AM imaging

Esposito, E. and Harris, J. and McConnell, G. (2007) Fast wavelength-tunable ultra-violet laser source for confocal Fura-2AM imaging. Optics Communications, 270 (2). pp. 470-473. ISSN 0030-4018

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Abstract

We report a novel wavelength-flexible laser source for three-dimensional ultra-violet imaging. Based on supercontinuum generation in photonic crystal fiber, the resultant broadband laser source extended from A = 331 nm into the visible region of the spectrum. Using an electronically-controlled filter wheel and filter set with a response time of approximately 50 ins, rapid wavelength selection was performed. The described scheme is capable of exciting the current range of ultra-violet-excited fluorophores and the simple and rapid wavelength control also provides a new approach for fast ratiometric imaging of Fura-2AM, facilitating an easy method of performing quantitative intracellular calcium concentration measurements.