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Fast wavelength-tunable ultra-violet laser source for confocal Fura-2AM imaging

Esposito, E. and Harris, J. and McConnell, G. (2007) Fast wavelength-tunable ultra-violet laser source for confocal Fura-2AM imaging. Optics Communications, 270 (2). pp. 470-473. ISSN 0030-4018

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Abstract

We report a novel wavelength-flexible laser source for three-dimensional ultra-violet imaging. Based on supercontinuum generation in photonic crystal fiber, the resultant broadband laser source extended from A = 331 nm into the visible region of the spectrum. Using an electronically-controlled filter wheel and filter set with a response time of approximately 50 ins, rapid wavelength selection was performed. The described scheme is capable of exciting the current range of ultra-violet-excited fluorophores and the simple and rapid wavelength control also provides a new approach for fast ratiometric imaging of Fura-2AM, facilitating an easy method of performing quantitative intracellular calcium concentration measurements.