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Receptor usage by the Acanthocheilonema viteae-derived immunomodulator, ES-62

Harnett, William and Goodridge, Helen and Allen, Janet and Harnett, Margaret (2012) Receptor usage by the Acanthocheilonema viteae-derived immunomodulator, ES-62. Experimental Parasitology. e.publication. ISSN 0014-4894 (In Press)

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    Abstract

    ES-62 is an immunomodulatory phosphorylcholine (PC)-containing glycoprotein secreted by the rodent filarial nematode Acanthocheilonema viteae. Previously, the use of knockout mice has revealed the effects of ES-62 on macrophages and dendritic cells to be dependent on TLR4. However, it is possible that ES-62 may interact with additional proteins on the surfaces of target cells and hence that cells may vary with respect to receptor usage. In this study, we identified by molecular weight, proteins that interact with ES-62 and found differences amongst the immune system cells studied. Thus, whereas lymphocytes appear to have two major interacting proteins of 135 and 82 kDa, U937 monocytes only contain an ES-62-binding protein of the latter molecular weight. Binding to the proteins on B cells and U937 cells wasblocked by PC, suggesting a critical role for this ES-62 moiety in facilitating interaction. Finally, ES-62 binding is followed by internalization in both macrophages and B cells but only in the former was absence of TLR4 found to block internalization. These findings are consistent with differences in receptor usage by ES-62 amongst different cell-types.

    Item type: Article
    ID code: 36284
    Keywords: acanthocheilonema, anthocheilonema viteae, filarial , immunomodulator , receptor usage, macrophage , protein, Therapeutics. Pharmacology, Immunology, Parasitology
    Subjects: Medicine > Therapeutics. Pharmacology
    Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
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    Depositing user: Pure Administrator
    Date Deposited: 01 Dec 2011 14:37
    Last modified: 27 Mar 2014 22:37
    URI: http://strathprints.strath.ac.uk/id/eprint/36284

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