Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Transient electrical field across cellular membranes - pulsed electric field treatment of microbial cells

Timoshkin, I. and MacGregor, S.J. and Fouracre, R.A. and Crichton, B.H. and Anderson, J.G. (2006) Transient electrical field across cellular membranes - pulsed electric field treatment of microbial cells. Journal of Physics D: Applied Physics, 39 (1). pp. 596-603. ISSN 0022-3727

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

The pulsed electric field (PEF) treatment of liquid and pumpable products contaminated with microorganisms has attracted significant interest from the pulsed power and bioscience research communities particularly because the inactivation mechanism is non-thermal, thereby allowing retention of the original nutritional and flavour characteristics of the product. Although the biological effects of PEF have been studied for several decades, the physical mechanisms of the interaction of the fields with microorganisms is still not fully understood. The present work is a study of the dynamics of the electrical field both in a PEF treatment chamber with dielectric barriers and in the plasma (cell) membrane of a microbial cell. It is shown that the transient process can be divided into three physical phases, and models for these phases are proposed and briefly discussed. The complete dynamics of the time development of the electric field in a spherical dielectric shell representing the cellular membrane is then obtained using an analytical solution of the Ohmic conduction problem. It was found that the field in the membrane reaches a maximum value that could be two orders of magnitude higher than the original Laplacian electrical field in the chamber, and this value was attained in a time comparable to the field relaxation time in the chamber. Thus, the optimal duration of the field during PEF treatment should be equal to such a time.

Item type: Article
ID code: 3593
Keywords: applied physics, electric field, plasma, pulsed electric field, microbial cells, Electrical engineering. Electronics Nuclear engineering, Physics, Surfaces, Coatings and Films, Acoustics and Ultrasonics, Electronic, Optical and Magnetic Materials, Condensed Matter Physics
Subjects: Technology > Electrical engineering. Electronics Nuclear engineering
Science > Physics
Department: Faculty of Engineering > Electronic and Electrical Engineering
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 11 Jun 2007
    Last modified: 04 Sep 2014 13:46
    URI: http://strathprints.strath.ac.uk/id/eprint/3593

    Actions (login required)

    View Item