Caro, Ester and Marcé, Rosa M. and Cormack, Peter A.G. and Sherrington, David C. and Borrull, Francesc (2006) Direct determination of ciprofloxacin by mass spectrometry after a two-step solid-phase extraction using a molecularly imprinted polymer. Journal of Separation Science, 29 (9). pp. 1230-1236. ISSN 1615-9306Full text not available in this repository. (Request a copy from the Strathclyde author)
A molecularly imprinted polymer (MIP) has been prepared for the first time with ciprofloxacin (CIPRO) as the template molecule, via a noncovalent synthetic procedure. Prior to its use as a sorbent in SPE, the MIP was evaluated chromatographically to confirm that it was indeed molecularly imprinted. The MIP was then used to extract CIPRO selectively from urine samples by means of a two-step SPE procedure in which a commercial Oasis cartridge and a molecularly imprinted SPE cartridge were combined in series. This approach allowed the matrix compounds present in the samples to be removed effectively. The urine extracts obtained after this two-step SPE procedure was applied were relatively clean compared to the original samples, and this made it possible to inject directly the extracts into a mass spectrometer and thus quantify CIPRO in urine samples at low levels and reduce the time of analysis.
|Keywords:||fluorinated quinolones, human urine, mass spectrometry, molecularly imprinted polymer, solid-phase extraction, Chemistry, Filtration and Separation, Analytical Chemistry|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry|
|Depositing user:||Pure Administrator|
|Date Deposited:||08 Nov 2011 16:54|
|Last modified:||22 Mar 2017 11:51|