Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Spectroscopic analysis of protein Fe-NO complexes.

Bellota-Anton, Cesar and Munnoch, John and Robb, Kirsty and Adamczyk, Katrin and Candelaresi, Marco and Parker, Anthony W. and Dixon, Ray and Hutchings, Matthew I. and Hunt, Neil and Tucker, Nicholas (2011) Spectroscopic analysis of protein Fe-NO complexes. Biochemical Society Transactions, 39. 1293 -1298. ISSN 0300-5127

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

The toxic free radical NO (nitric oxide) has diverse biological roles in eukaryotes and bacteria, being involved in signalling, vasodilation, blood clotting and immunity, and as an intermediate in microbial denitrification. The predominant biological mechanism of detecting NO is through the formation of iron nitrosyl complexes, although this is a deleterious process for other iron-containing enzymes. We have previously applied techniques such as UV–visible and EPR spectroscopy to the analysis of protein Fe–NO complex formation in order to study how NO controls the activity of the bacterial transcriptional regulators NorR and NsrR. These studies have analysed NO-dependent biological activity both in vitro and in vivo using diverse biochemical, molecular and spectroscopic methods. Recently, we have applied ultrafast 2D-IR (two-dimensional IR) spectroscopy to the analysis of NO–protein interactions using Mb (myoglobin) and Cc (cytochrome c) as model haem proteins. The ultrafast fluctuations of Cc and Mb show marked differences, indicating altered flexibility of the haem pockets. We have extended this analysis to bacterial catalase enzymes that are known to play a role in the nitrosative stress response by detoxifying peroxynitrite. The first 2D-IR analysis of haem nitrosylation and perspectives for the future are discussed.

Item type: Article
ID code: 35461
Keywords: spectroscopic analysis, protein, nitric oxide, free radical, iron nitrosyl complexes, TIC - Bionanotechnology, Biochemistry
Subjects: UNSPECIFIED
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Physics
Technology and Innovation Centre > Bionanotechnology
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 31 Oct 2011 15:56
    Last modified: 05 Sep 2014 13:14
    URI: http://strathprints.strath.ac.uk/id/eprint/35461

    Actions (login required)

    View Item