Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Biocatalytic self-assembly of 2D peptide-based nanostructures

Hughes, M. and Xu, haixia and Frederix, Pim and Smith, A.M. and Hunt, Neil and Tuttle, Tell and Kinloch, I.A. and Ulijn, Rein Vincent (2011) Biocatalytic self-assembly of 2D peptide-based nanostructures. Soft Matter, 7 (21). pp. 10032-10038. ISSN 1744-683X

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Peptide based 2D nanostructures of micronscale size in both X and Y dimensions are extremely rare because amino acid chirality favours helical structures, and nucleation-growth mechanisms usually favour uni-directional growth. We demonstrate the production of extended two-dimensional (2D) peptide nanostructures via the thermolysin catalysed condensation of Fmoc protected hydrophilic amino acid (serine, Fmoc-S) and a hydrophobic amino acid ester (phenylalanine, F-OMe). We propose that lateral self-assembly is enabled by the reversible nature of the system, favouring the thermodynamic product (extended sheets) over kinetically favoured 1 dimensional structures. Fmoc-SF-OMe forms extended arrays of β-sheet structures interlock via π-stacking between Fmoc groups. We propose that, due to its alternating hydrophilic/hydrophobic amino acid sequence, amphiphilic sheets presenting either phenyl or hydroxyl functionality are formed that assemble pair-wise, thereby shielding hydrophobic groups from the aqueous environment. Formation of these structures was supported by fluorescence emission spectroscopy, FTIR and XRD analysis and molecular mechanics minimization. At enhanced enzyme concentrations, hierarchical self-assembly was observed giving rise to spherulitic structures, with the number of spherulites dictated by enzyme concentration.

Item type: Article
ID code: 35452
Keywords: peptide-based nanostructures , nanostructures , spherulites, TIC - Bionanotechnology, Chemistry, Physics, Chemistry(all), Condensed Matter Physics
Subjects: Science > Chemistry
Science > Physics
Department: Faculty of Science > Physics
Technology and Innovation Centre > Bionanotechnology
Faculty of Science > Pure and Applied Chemistry
Related URLs:
Depositing user: Pure Administrator
Date Deposited: 31 Oct 2011 14:51
Last modified: 27 Mar 2014 09:47
URI: http://strathprints.strath.ac.uk/id/eprint/35452

Actions (login required)

View Item