Picture of virus under microscope

Research under the microscope...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

Explore SIPBS research

Two-photon excited surface plasmon enhanced energy transfer between DAPI and gold nanoparticles: opportunities in intra-cellular imaging and sensing

Zhang, Yinan and Birch, David J. S. and Chen, Yu (2011) Two-photon excited surface plasmon enhanced energy transfer between DAPI and gold nanoparticles: opportunities in intra-cellular imaging and sensing. Applied Physics Letters, 99 (10). -. ISSN 0003-6951

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

We have demonstrated energy transfer between 4'-6-Diamidino-2-phenylindole (DAPI), a commonly used DNA label, and gold nanoparticles under two-photon excitation in solution using fluorescence lifetime imaging microscopy (FLIM). With comparable size and concentration, gold nanorods (GNRs) are shown to provide more efficient energy transfer than gold nanospheres (GNSs). We attribute this transfer enhancement effect to the longitudinal surface plasmon mode of GNRs overlapping with the excitation wavelength. Energy transfer under two-photon excitation between GNRs and DAPI has also been observed in cell culture and found to be in accord with the solution phase results.