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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

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RNA stability in frozen/thawed clinical blood samples

Posada Estefan, Olga Maria and Tate, Rothwelle and Grant, Mary (2011) RNA stability in frozen/thawed clinical blood samples. In: British Toxicology Society Meeting, 2011-09-05 - 2011-09-06.

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Abstract

The main challenge of RNA extraction from frozen blood samples is to obtain sufficient RNA of acceptable quality for gene expression analysis. In this trial we compared the RNA stability in clinical blood samples with and without a RNA stabilizer, RNAlater. Results show that high quality RNA can be extracted from blood with RNAlater that has been frozen and thawed several times before the extraction.