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RNA stability in frozen/thawed clinical blood samples

Posada Estefan, Olga Maria and Tate, Rothwelle and Grant, Mary (2011) RNA stability in frozen/thawed clinical blood samples. In: British Toxicology Society Meeting, 2011-09-05 - 2011-09-06.

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Abstract

The main challenge of RNA extraction from frozen blood samples is to obtain sufficient RNA of acceptable quality for gene expression analysis. In this trial we compared the RNA stability in clinical blood samples with and without a RNA stabilizer, RNAlater. Results show that high quality RNA can be extracted from blood with RNAlater that has been frozen and thawed several times before the extraction.