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Clustered integrin α5β1 ligand displays model fibronectin-mediated adhesion of human endometrial stromal cells

Li, Zhaohui and Kreiner, Michaela and van der Walle, Christopher F and Mardon, Helen J (2011) Clustered integrin α5β1 ligand displays model fibronectin-mediated adhesion of human endometrial stromal cells. Biochemical and Biophysical Research Communications, 407 (4). pp. 777-782.

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Abstract

Progress towards endometrial tissue engineering for modelling endometrial diseases and infertility is frustrated by the inability to mimic the fibronectin (FN) extracellular matrix required by human endometrial stromal cells (EnSCs). Here we show that this is because of the requirement to present integrin α5β1 (the FN receptor) ligands in specifically oriented, polyvalent displays; by engineering controlled self-assembly of the 9th-10th type III FN domain pair (FIII9-10, the minimal integrin α5β1 ligand) immobilised in a specific orientation to cell culture surfaces. The fraction of adherent EnSCs seen to spread increased significantly for the multimeric ligand surfaces in the order: tetramer>trimer>dimer>monomer. The extent of EnSC spread morphology also increased in the same order, with the tetrameric ligand supporting a morphology most similar to that supported by FN. Our data suggest that only higher-order multimers of FIII9-10 will fully promote cell spreading mediated through integrin α5β1 binding.

Item type: Article
ID code: 35154
Notes: Copyright © 2011 Elsevier Inc. All rights reserved.
Keywords: cell adhesion, endometrium, female, fibronectins, humans, Integrin alpha5beta1, ligands, stromal cells, tissue engineering, Pharmacy and materia medica, Biochemistry, Cell Biology, Molecular Biology, Biophysics
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
Depositing user: Pure Administrator
Date Deposited: 25 Oct 2011 16:46
Last modified: 27 Mar 2014 09:45
URI: http://strathprints.strath.ac.uk/id/eprint/35154

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