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Analysis of ketamine and norketamine in hair samples using molecularly imprinted solid-phase extraction (MISPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Harun, Norlida and Anderson, Robert A. and Cormack, Peter A. G. (2010) Analysis of ketamine and norketamine in hair samples using molecularly imprinted solid-phase extraction (MISPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analytical and bioanalytical chemistry, 396 (7). pp. 2449-2459. ISSN 1618-2642

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Abstract

An anti-ketamine molecularly imprinted polymer (MIP) was synthesized and used as the sorbent in a solid-phase extn. protocol to isolate ketamine and norketamine from human hair exts. prior to LC-MS/MS anal. Under optimized conditions, the MIP was capable of selectively rebinding ketamine, a licensed anesthetic that is widely misused as a recreational drug, with an apparent binding capacity of 0.13 μg ketamine per mg polymer. The limit of detection (LOD) and lower limit of quantification (LLOQ) for both ketamine and norketamine were 0.1 ng/mg hair and 0.2 ng/mg hair, resp., when 10 mg hair were analyzed. The method was linear from 0.1 to 10 ng/mg hair, with correlation coeffs. (R2) of better than 0.99 for both ketamine and norketamine. Recoveries from hair samples spiked with ketamine and norketamine at a concn. of 50 ng/mg were 86% and 88%, resp. The method showed good intra- and interday precisions.

Item type: Article
ID code: 34994
Notes: M1 - Copyright (C) 2011 American Chemical Society (ACS). All Rights Reserved. CAPLUS AN 2010:69525(Journal)
Keywords: liquid chromatography , ketamine , norketamine, bioanalytical chemistry, Chemistry, Physical and theoretical chemistry, Biochemistry, Analytical Chemistry
Subjects: Science > Chemistry
Science > Chemistry > Physical and theoretical chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
Depositing user: Pure Administrator
Date Deposited: 21 Oct 2011 14:57
Last modified: 27 Mar 2014 09:45
URI: http://strathprints.strath.ac.uk/id/eprint/34994

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