Picture of smart phone in human hand

World leading smartphone and mobile technology research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by Strathclyde researchers from the Department of Computer & Information Sciences involved in researching exciting new applications for mobile and smartphone technology. But the transformative application of mobile technologies is also the focus of research within disciplines as diverse as Electronic & Electrical Engineering, Marketing, Human Resource Management and Biomedical Enginering, among others.

Explore Strathclyde's Open Access research on smartphone technology now...

Isolation and characterization of insulin-stimulated, high-affinity cAMP phosphodiesterases from rat liver

Houslay, M D and Pyne, N J and Cooper, M E (1988) Isolation and characterization of insulin-stimulated, high-affinity cAMP phosphodiesterases from rat liver. Methods in Enzmology, 159. pp. 751-760. ISSN 0076-6879

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

The ability of glucagon to elevate intracellular cAMP concentrations in hepatocytes is antagonized by insulin.l This effect is achieved through inhibition of adenylate cyclasC and activation of specific cAMP phosphodiesterases. l,3,4 The activation of two specific cAMP phosphodiesterases can readily be assessed using a rapid Percoll gradient fractionation procedure to resolve the various membrane fractions. 1 Both of these enzymes express a high affinity and specificity for cAMP. One of them is a peripheral protein found associated exclusively with the plasma membrane 5 and is activated by its phosphorylation, which is triggered by insulin. 6 The other is associated with an as yet undefined intracellular dense-vesicle fraction.~ The mechanism of its activation has yet to be defined but may involve membrane processing/translocation reactions. 7 The dense-vesicle enzyme can also be activated by glucagon through a process which is distinct from that whereby insulin elicits its activation.