Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Investigation into the concanavalin A reactivity, fucosylation and oligosaccharide microheterogeneity of alpha(1)-acid glycoprotein expressed in the sera of patients with rheumatoid arthritis

Elliott, M A and Elliott, H G and Gallagher, K and McGuire, J and Field, M and Smith, K D (1997) Investigation into the concanavalin A reactivity, fucosylation and oligosaccharide microheterogeneity of alpha(1)-acid glycoprotein expressed in the sera of patients with rheumatoid arthritis. Journal of chromatography. B, Biomedical sciences and applications, 688 (2). pp. 229-237. ISSN 1387-2273

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

alpha(1)-Acid glycoprotein (AGP) exists as an heterogeneous population of glycosylated variants (glycoforms) in plasma. The concentration of AGP increases some 2-5 fold in certain pathophysiological states exemplified by the chronic inflammatory disease, rheumatoid arthritis (RA). Moreover, the expressed glycosylation pattern alters in such conditions, indicating functional significance that is likely to be related to the oligosaccharide heterogeneity. We have investigated the heterogeneity of AGP glycosylation using the technique of high pH anion-exchange chromatography (HPAEC). AGP was isolated from the blood of RA sufferers, partially separated by Concanavalin A (Con A) affinity chromatography into bound and non-bound fractions and was enzymatically deglycosylated. Chromatography on the pellicular HPAE resin at pH 13 separated the released oligosaccharides and allowed a comparison of profiles in terms of branching and fucosylation. Results demonstrate an abnormal RA AGP glycosylation, with a tendency towards tri- and tetra-antennary oligosaccharides and enhanced fucosylation, in addition to the possible existence of penta-sialylated RA AGP glycoforms.

Item type: Article
ID code: 34867
Keywords: alpha(1)-acid glycoprotein, anion-exchange chromatography, pulsed amperometric detection, glycoproteins, inflammation, Therapeutics. Pharmacology, Chemistry(all)
Subjects: Medicine > Therapeutics. Pharmacology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 11 Nov 2011 16:15
    Last modified: 05 Sep 2014 12:27
    URI: http://strathprints.strath.ac.uk/id/eprint/34867

    Actions (login required)

    View Item