Picture of person typing on laptop with programming code visible on the laptop screen

World class computing and information science research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

Explore

Investigation into the concanavalin A reactivity, fucosylation and oligosaccharide microheterogeneity of alpha(1)-acid glycoprotein expressed in the sera of patients with rheumatoid arthritis

Elliott, M A and Elliott, H G and Gallagher, K and McGuire, J and Field, M and Smith, K D (1997) Investigation into the concanavalin A reactivity, fucosylation and oligosaccharide microheterogeneity of alpha(1)-acid glycoprotein expressed in the sera of patients with rheumatoid arthritis. Journal of chromatography. B, Biomedical sciences and applications, 688 (2). pp. 229-237. ISSN 1387-2273

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

alpha(1)-Acid glycoprotein (AGP) exists as an heterogeneous population of glycosylated variants (glycoforms) in plasma. The concentration of AGP increases some 2-5 fold in certain pathophysiological states exemplified by the chronic inflammatory disease, rheumatoid arthritis (RA). Moreover, the expressed glycosylation pattern alters in such conditions, indicating functional significance that is likely to be related to the oligosaccharide heterogeneity. We have investigated the heterogeneity of AGP glycosylation using the technique of high pH anion-exchange chromatography (HPAEC). AGP was isolated from the blood of RA sufferers, partially separated by Concanavalin A (Con A) affinity chromatography into bound and non-bound fractions and was enzymatically deglycosylated. Chromatography on the pellicular HPAE resin at pH 13 separated the released oligosaccharides and allowed a comparison of profiles in terms of branching and fucosylation. Results demonstrate an abnormal RA AGP glycosylation, with a tendency towards tri- and tetra-antennary oligosaccharides and enhanced fucosylation, in addition to the possible existence of penta-sialylated RA AGP glycoforms.