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Modulation of sialyl lewis X dependent binding to e-selectin by glycoforms of alpha-1-acid glycoprotein expressed in rheumatoid arthritis

Jorgensen, H G and Elliott, M A and Priest, R and Smith, K D (1998) Modulation of sialyl lewis X dependent binding to e-selectin by glycoforms of alpha-1-acid glycoprotein expressed in rheumatoid arthritis. Biomedical Chromatography, 12 (6). pp. 343-349. ISSN 0269-3879

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Abstract

Alpha-1-acid glycoprotein (AGP) is an extensively glycosylated acute phase protein of imprecisely defined physiological function. Nonetheless it is known that the oligosaccharide component comprising 42% of the 41 kDa molecular weight is critical to the previously described multifarious immunomodulatory functions of AGP in vitro. Complex oligosaccharides were enzymically released from AGP purified from the blood of rheumatoid arthritis sufferers by our oligosaccharide protective method. Oligosaccharide profiling was by means of high pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Monosaccharide composition analysis revealed increased fucosylation of inflammatory AGP oligosaccharide chains, suggesting the potential for expression of the tetrasaccharide antigen and E-Selectin ligand, sialyl Lewis X (sLeX), The hypothesis that AGP may function to inhibit blood cell binding to activated endothelium at E-Selectin was tested in a microtitre cell-protein binding assay. In this system we have shown that the oligosaccharide moiety of AGP, as expressed in inflammatory disease, can inhibit the sLeX/E-Selectin interaction. Thus we have identified a correlation between the abnormal glycosylation of AGP in rheumatoid arthritis and suppression of sLeX dependent cell adhesion through inhibition of E-selectin binding which could be the basis of a novel, site specific, anti-inflammatory agent. (C) 1998 John Wiley & Sons, Ltd.

Item type: Article
ID code: 34863
Keywords: alpha(1)-acid glycoprotein, plasma, microheterogeneity, Biochemistry, Drug Discovery, Molecular Biology, Analytical Chemistry, Pharmacology, Clinical Biochemistry
Subjects: UNSPECIFIED
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 07 Nov 2011 16:57
    Last modified: 05 Sep 2014 12:26
    URI: http://strathprints.strath.ac.uk/id/eprint/34863

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