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Use of mathematical derivatives (time-domain differentiation) on chromatographic data to enhance the detection and quantification of an unknown 'rider' peak

Ford, S J and Elliott, M A and Halbert, G W (2003) Use of mathematical derivatives (time-domain differentiation) on chromatographic data to enhance the detection and quantification of an unknown 'rider' peak. Journal of Pharmaceutical and Biomedical Analysis, 33 (4). pp. 563-570. ISSN 0731-7085

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Abstract

Two samples of an anticancer prodrug, AQ4N, were submitted for HPLC assay and showed an unidentified impurity that eluted as a 'rider' on the tail of the main peak. Mathematical derivatization of the chromatograms offered several advantages over conventional skimmed integration. A combination of the second derivative amplitude and simple linear regression gave a novel method for estimating the true peak area of the impurity peak. All the calculation steps were carried out using a widely available spreadsheet program. (C) 2003 Elsevier B.V. All rights reserved.

Item type: Article
ID code: 34853
Keywords: tangent integration, skimmed integration, resolution enhancement, baseline prediction, liquid-chromatography, quantitation, errors, AQ4N, Pharmacy and materia medica, Drug Discovery, Pharmaceutical Science, Spectroscopy, Analytical Chemistry, Clinical Biochemistry
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Pure Administrator
Date Deposited: 27 Jan 2012 11:06
Last modified: 18 Jun 2015 04:34
URI: http://strathprints.strath.ac.uk/id/eprint/34853

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