Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Impaired Gluconeogenesis in a porcine model of paracetamol induced acute liver failure

Dabos, Konstantinos and Whalen, Henry and Newsome, Philip and Parkinson, John and Henderson, Neil and Sadler, Ian and Hayes, Peter and Plevris, John (2011) Impaired Gluconeogenesis in a porcine model of paracetamol induced acute liver failure. World Journal of Gastroenterology, 17 (11). pp. 1457-1461. ISSN 1007-9327

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF). Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using (1)H NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples. No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05). Our pig model of ALF is characterized by an altered gluconeogenetic capacity, an impaired tricarboxylic acid (TCA) cycle and a glycolytic state.

Item type: Article
ID code: 34141
Keywords: lactate, pyruvate, branch chain amino acids, aromatic amino acids, Science, Gastroenterology
Subjects: Science
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 20 Oct 2011 10:25
    Last modified: 05 Sep 2014 11:35
    URI: http://strathprints.strath.ac.uk/id/eprint/34141

    Actions (login required)

    View Item