Dennany, L and Forster, R J and White, B and Smyth, M and Rusling, J F (2004) Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+). Journal of the American Chemical Society, 126 (28). pp. 8835-8841. ISSN 0002-7863Full text not available in this repository. (Request a copy from the Strathclyde author)
Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.
|Keywords:||mediated fenton reactions, hydrogen-peroxide, oxidative stress, oxygen radicals, electrochemical oxidation, strand breaks, in-vivo, iron, Chemistry, Biochemistry, Colloid and Surface Chemistry, Chemistry(all), Catalysis|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry|
|Depositing user:||Pure Administrator|
|Date Deposited:||20 Oct 2011 12:46|
|Last modified:||02 Dec 2016 03:43|