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Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)

Dennany, L and Forster, R J and White, B and Smyth, M and Rusling, J F (2004) Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+). Journal of the American Chemical Society, 126 (28). pp. 8835-8841. ISSN 0002-7863

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Abstract

Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.