Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Simultaneous direct electrochemiluminescence and catalytic voltammetry detection of DNA in ultrathin films

Dennany, L and Forster, R J and Rusling, J F (2003) Simultaneous direct electrochemiluminescence and catalytic voltammetry detection of DNA in ultrathin films. Journal of the American Chemical Society, 125 (17). pp. 5213-5218. ISSN 0002-7863

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Direct electrochemiluminescence (ECL) involving DNA was demonstrated in 10 nm films of cationic polymer [Ru(bpy)(2)(PVP)(10)](2+) assembled layer-by-layer with DNA. A square wave voltammetric waveform oxidized the Ru-II sites in the metallopolymer to Ru-III, and ECL was measured simultaneously with catalytic voltammetric peaks in a simple apparatus. Significant ECL generation occurred only when guanine bases were present on oligonucleotides in the films. This result along with knowledge of proposed ECL pathways suggests that guanine radicals initially formed by catalytic oxidation of guanines by Ru-III react with the metallopolymer to produce electronically exited Ru-II* sites in the film. ECL and catalytic SWV peaks were sensitive to oligonucleotide hybridization and chemical DNA damage. Simultaneous linear growth of ECL and SWV peaks occurred after incubation with known DNA damage agent styrene oxide over 20 min. The estimated detection limit was 1 damaged DNA base in 1000. Control incubations of metallopolymer/dsDNA films in buffer containing unreactive toluene resulted in no significant changes of the ECL or SWV peaks.

Item type: Article
ID code: 34054
Keywords: electrogenerated chemiluminescence, guanine electron-transfer, square-wave voltammetry, oxidation, hybridization, kinetics, adducts, assays, Chemistry, Biochemistry, Colloid and Surface Chemistry, Chemistry(all), Catalysis
Subjects: Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 20 Oct 2011 13:48
    Last modified: 05 Sep 2014 11:28
    URI: http://strathprints.strath.ac.uk/id/eprint/34054

    Actions (login required)

    View Item