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A solvent-free matrix application method for matrix-assisted laser desorption/ionization imaging of small molecules

Goodwin, R J and Macintyre, L and Watson, D G and Scullion, S P and Pitt, A R (2010) A solvent-free matrix application method for matrix-assisted laser desorption/ionization imaging of small molecules. Rapid Communications in Mass Spectrometry, 24 (11). pp. 1682-1686. ISSN 1097-0231

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Abstract

Matrix application continues to be a critical step in sample preparation for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI). Imaging of small molecules such as drugs and metabolites is particularly problematic because the commonly used washing steps to remove salts are usually omitted as they may also remove the analyte, and analyte spreading is more likely with conventional wet matrix application methods. We have developed a method which uses the application of matrix as a dry, finely divided powder, here referred to as dry matrix application, for the imaging of drug compounds. This appears to offer a complementary method to wet matrix application for the MALDI-MSI of small molecules, with the alternative matrix application techniques producing different ion profiles, and allows the visualization of compounds not observed using wet matrix application methods. We demonstrate its value in imaging clozapine from rat kidney and 4-bromophenyl-1,4-diazabicyclo(3.2.2)nonane-4-carboxylic acid from rat brain. In addition, exposure of the dry matrix coated sample to a saturated moist atmosphere appears to enhance the visualization of a different set of molecules.

Item type: Article
ID code: 33842
Notes: Copyright (c) 2010 John Wiley & Sons, Ltd.
Keywords: animals, bicyclo compounds, heterocyclic, brain chemistry, clozapine, kidney, male, rats, rats, inbred BB, rats, wistar, spectrometry, mass, matrix-assisted laser desorption-ionization, Pharmacy and materia medica, Organic Chemistry, Spectroscopy, Analytical Chemistry
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
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Depositing user: Pure Administrator
Date Deposited: 11 Oct 2011 15:47
Last modified: 27 Mar 2014 09:37
URI: http://strathprints.strath.ac.uk/id/eprint/33842

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