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The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

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Cytochrome b or cytochrome c oxidase subunit I for mammalian species identification—An answer to the debate

Tobe, Shanan S. and Kitchener, Andrew and Linacre, Adrian (2009) Cytochrome b or cytochrome c oxidase subunit I for mammalian species identification—An answer to the debate. Forensic Science International: Genetics Supplement Series, 2 (1). pp. 306-307. ISSN 1875-1768

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Abstract

Species identification for forensic purposes is being increasingly used, as the value of non-human evidence is realized. This requires the identification of the species before individual analysis can take place. Traditionally the cytochrome b (cyt b) gene was used for species identification, but in 2003 the cytochrome c oxidase subunit 1 (CO1) gene was introduced under the terminology ‘barcoding’. This started an ongoing debate as to which gene offers the best template for species identification (high inter-species variability and low intra-species variation). Sequence data from 236 mammals were compared with multiple sequence alignments for a large number of human, cow and dog samples. Comparisons were made based on the number of inter-species variations between the different species and the intra-species variation between members of the same species.