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Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Identifying endangered species from degraded mixtures at low levels

Tobe, Shanan S. and Linacre, Adrian (2009) Identifying endangered species from degraded mixtures at low levels. Forensic Science International: Genetics Supplement Series, 2 (1). pp. 304-305. ISSN 1875-1768

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Abstract

Although endangered species are afforded protection under national and international laws, trade in products containing endangered species is still one of the most lucrative criminal enterprises in the world. Forensic science is employed to determine if endangered species are present in commercial products but runs into problems due to the extremely low levels of DNA and degraded nature of the majority of samples. Therefore a section of the mitochondrial genome is generally amplified and sequenced to determine if endangered species are present. This technique is sensitive and accurate, but cannot identify components of a DNA mixture due to the use of universal primers and can fail with subcellular levels of DNA. A multiplex PCR was therefore developed, based on the cytochrome b and 12S rRNA genes, to identify the presence of endangered mammalian species, if present, in commercial products. Four species [tiger (Panthera tigris ssp.), leopard (Panthera pardus), musk deer (Moschus sp.) and Asiatic black bear (Ursus thibetanus ssp.)] in addition to several common non-protected mammalian species can be identified using species-specific primers. A specific PCR product is produced depending on which species is/are present. The test is accurate and sensitive to low levels of DNA. It has been tested using traditional East Asian Medicine claiming to contain one or more endangered mammalian species. The test can be performed using standard DNA analysis techniques and implemented by any laboratory with these facilities.