Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Stable dye-labelled oligonucleotide-nanoparticle conjugates for nucleic acid detection

Barrett, Lee and Dougan, Jennifer A. and Faulds, Karen and Graham, Duncan (2011) Stable dye-labelled oligonucleotide-nanoparticle conjugates for nucleic acid detection. Nanoscale, 3 (8). pp. 3221-3227. ISSN 2040-3364

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Metallic nanoparticles functionalized with oligonucleotides are used for a number of nucleic acid detection strategies. However, oligonucleotide-nanoparticle conjugates suffer from a lack of stability when exposed to certain conditions associated with DNA detection assays. In this study, we report the synthesis of thiol and thioctic acid-modified oligonucleotide gold nanoparticle (OGNs) conjugates functionalized with a dye label and varying spacer groups. The thioctic acid-modified conjugates exhibit increased stability when treated with dithiothreitol (DTT) compared to the more commonly used thiol modification. When the dye labelled oligonucleotide nanoparticle conjugates are exposed to the same conditions there is a pronounced increase in the stability for both thioctic acid and thiol modified sequences. These results open up the possibility of simply using a dye label to enhance the stability of oligonucleotide-nanoparticle conjugates in DNA detection assays where the enhanced stability of the conjugate system can be advantageous in more complex biological environments.

Item type: Article
ID code: 33644
Keywords: raman scattering , nanoparicles, polymerase chain reaction, bound primers, dna, probes, serrs, TIC - Bionanotechnology, Physical and theoretical chemistry, Materials Science(all)
Subjects: Science > Chemistry > Physical and theoretical chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Technology and Innovation Centre > Bionanotechnology
Related URLs:
Depositing user: Pure Administrator
Date Deposited: 17 Oct 2011 14:04
Last modified: 27 Mar 2014 09:35
URI: http://strathprints.strath.ac.uk/id/eprint/33644

Actions (login required)

View Item