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Accurate determination of adjuvant-associated protein or peptide by ninhydrin assay

Brewer, J M and Roberts, C W and Stimson, W H and Alexander, J (1995) Accurate determination of adjuvant-associated protein or peptide by ninhydrin assay. Vaccine, 13 (15). pp. 1441-1444. ISSN 0264-410X

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Abstract

Modern peptide and subunit vaccines are increasingly having to rely on the use of immunological adjuvants to achieve effective immunity. However, the only adjuvant currently approved for use in humans is aluminium hydroxide, although many adjuvants are currently under preclinical development. Determining immunogen concentration in the presence of adjuvants such as aluminium hydroxide gel, liposomes or NISV has proved to be problematic. One approach has been to use radiolabelled antigens to extrapolate concentration to a preparation using native immunogen. However, the use of a colorimetric assay would allow greater flexibility in terms of immunogen used and would reduce costs and remove safety problems. Of the colorimetric methods we have examined thus far, only the manual ninhydrin assay has produced consistent results with detection of microgram quantities of protein or peptide in the presence of NISV or Alhydrogel, but not liposomes. As the assay relies on the detection of free amino groups after protein hydrolysis, peptides as well as proteins may be effectively determined irrespective of amino acid composition, a considerable advantage over other colorimetric assay systems.

Item type: Article
ID code: 33633
Keywords: adjuvants, immunologic, aluminum hydroxide, liposomes, measles vaccine, ninhydrin, ovalbumin, peptides, surface-active agents, viral proteins, Pharmacy and materia medica, Infectious Diseases, Molecular Medicine, Public Health, Environmental and Occupational Health, veterinary(all), Immunology and Microbiology(all)
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 28 Sep 2011 16:38
    Last modified: 05 Sep 2014 11:01
    URI: http://strathprints.strath.ac.uk/id/eprint/33633

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