Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

DNA vaccination with the immunodominant tachyzoite surface antigen (SAG-1) protects against adult acquired Toxoplasma gondii infection but does not prevent maternofoetal transmission

Couper, Kevin N and Nielsen, Henrik V and Petersen, Eskild and Roberts, Fiona and Roberts, Craig W and Alexander, James (2003) DNA vaccination with the immunodominant tachyzoite surface antigen (SAG-1) protects against adult acquired Toxoplasma gondii infection but does not prevent maternofoetal transmission. Vaccine, 21 (21-22). pp. 2813-2820. ISSN 0264-410X

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

We examined the ability of a DNA vaccine comprising the Toxoplasma gondii tachyzoite immunodominant surface antigen, SAG-1, to both protect adult BALB/c mice against infection with the avirulent Beverly type-2 strain of T. gondii and also to inhibit the incidence of congenital disease. Vaccination induced an enhanced type-1 immune response as measured by IgG2a antibody production and increased splenocyte IFN-gamma production. Vaccination also limited disease following infection via either the oral or peritoneal routes as measured by mortality, pathology or brain cyst burden. While vaccination with plasmid alone also increased splenocyte IFN-gamma production, this afforded no protection and following infection mortality rates and cyst burden counts were similar in this group to that of non-vaccinated animals. Although, vaccination with SAG-1 DNA did protect against adult acquired T. gondii infection, it did not prevent maternofoetal transmission in previously vaccinated dams infected during pregnancy. The results indicate differences in the protective mechanisms operating between adult acquired disease and congenital transmission and have significant implications for future vaccine development.

Item type: Article
ID code: 33626
Keywords: animals, animals, newborn, antigens, protozoan, antigens, surface, female, infectious disease transmission, vertical, injections, intraperitoneal, maternal-fetal exchange, mice, mice, inbred BALB C, pregnancy, protozoan proteins, time factors, toxoplasmosis, animal, vaccination, vaccines, DNA, Pharmacy and materia medica, Infectious Diseases, Molecular Medicine, Public Health, Environmental and Occupational Health, veterinary(all), Immunology and Microbiology(all)
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 17 Nov 2011 10:57
    Last modified: 05 Sep 2014 11:00
    URI: http://strathprints.strath.ac.uk/id/eprint/33626

    Actions (login required)

    View Item