Marenah, Lamin and Allen, Elaine and Mountford, Joanne and Holyoake, Tessa and Jorgensen, Heather and Elliott, Moira (2012) Investigation into omacetaxine solution stability for in vitro study. Biomedical Chromatography, 26 (5). pp. 545-547. ISSN 0269-3879Full text not available in this repository. (Request a copy from the Strathclyde author)
Omacetaxine is a natural product extract originating from Chinese medicine and finding therapeutic use as a potent myelosuppressive agent in leukemia. When planning in vitro cell biology experiments to assess omacetaxine activity against primary leukemic stem cells, it became apparent that the literature rarely describes the in vitro stability of the molecule, although accessible chromatographic methods have been published. Clearly whole organisms vs their component cells will differ in the way in which they handle xenobiotics, with the latter more dependent on physiochemical parameters such as pH and temperature in the absence of active metabolism or excretion. This could impact on the cells’ experience of drug in culture. We therefore report here on examination of a modified, high-performance liquid chromatography (HPLC) method with assessment of degradant production from a 72 h solution stability study, clearly demonstrating that omacetaxine is highly stable in representative cell culture conditions (37 °C, neutral pH) and persists for many days in marked contrast to its short-half life in vivo.
|Keywords:||omacetaxine solution stability , myelosuppressive agent , leukemia, Therapeutics. Pharmacology, Biochemistry, Drug Discovery, Molecular Biology, Analytical Chemistry, Pharmacology, Clinical Biochemistry|
|Subjects:||Medicine > Therapeutics. Pharmacology|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Pure Administrator|
|Date Deposited:||12 Aug 2011 10:54|
|Last modified:||06 Jan 2017 09:51|