Bryson, Karen and Millington, Owain and Mokgethi, Thabang and McGachy, Adrienne and Brombacher, Frank and Alexander, James (2011) BALB/c mice deficient in CD4(+) T cell IL-4R alpha expression control Leishmania mexicana load although female but not male mice develop a healer phenotype. PLoS Neglected Tropical Diseases, 5 (1). pp. 1-10. ISSN 1935-2727
Immunologically intact BALB/c mice infected with Leishmania mexicana develop non-healing progressively growing lesions associated with a biased Th2 response while similarly infected IL-4R alpha-deficient mice fail to develop lesions and develop a robust Th1 response. In order to determine the functional target(s) for IL-4/IL-13 inducing non-healing disease, the course of L. mexicana infection was monitored in mice lacking IL-4R alpha expression in specific cellular compartments. A deficiency of IL-4R alpha expression on macrophages / neutrophils (in LysM(cre)IL-4R alpha(-lox) animals) had minimal effect on the outcome of L. mexicana infection compared with control (IL-4R alpha(-/flox)) mice. In contrast, CD4(+) T cell specific (Lys(cre)IL-4R alpha(-lox)) IL-4R alpha(-/-) mice infected with L. mexicana developed small lesions, which subsequently healed in female mice, but persisted in adult male mice. While a strong Th1 response was manifest in both male and female CD4(+) T cell specific IL-4R alpha(-/-) mice infected with L. mexicana, induction of IL-4 was manifest in males but not females, independently of CD4(+) T cell IL-4 responsiveness. Similar results were obtained using pan-T cell specific (iLck(cre)IL-4R alpha(-/lox)) IL-4R alpha(-/-) mice. Collectively these data demonstrate that upon infection with L. mexicana, initial lesion growth in BALB/c mice is dependent on non-T cell population(s) responsive to IL-4/IL-13 while progressive infection is dependent on CD4(+) T cells responsive to IL-4.
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