Picture of wind turbine against blue sky

Open Access research with a real impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

The Energy Systems Research Unit (ESRU) within Strathclyde's Department of Mechanical and Aerospace Engineering is producing Open Access research that can help society deploy and optimise renewable energy systems, such as wind turbine technology.

Explore wind turbine research in Strathprints

Explore all of Strathclyde's Open Access research content

BALB/c mice deficient in CD4(+) T cell IL-4R alpha expression control Leishmania mexicana load although female but not male mice develop a healer phenotype

Bryson, Karen and Millington, Owain and Mokgethi, Thabang and McGachy, Adrienne and Brombacher, Frank and Alexander, James (2011) BALB/c mice deficient in CD4(+) T cell IL-4R alpha expression control Leishmania mexicana load although female but not male mice develop a healer phenotype. PLOS Neglected Tropical Diseases, 5 (1). pp. 1-10. ISSN 1935-2727

[img]
Preview
PDF (Bryson Millington Mokgethietal PLoS 2011)
BrysonMillingtonMokgethietalPLoS2011.pdf - Published Version
Available under License Creative Commons Attribution.

Download (823kB) | Preview

Abstract

Immunologically intact BALB/c mice infected with Leishmania mexicana develop non-healing progressively growing lesions associated with a biased Th2 response while similarly infected IL-4R alpha-deficient mice fail to develop lesions and develop a robust Th1 response. In order to determine the functional target(s) for IL-4/IL-13 inducing non-healing disease, the course of L. mexicana infection was monitored in mice lacking IL-4R alpha expression in specific cellular compartments. A deficiency of IL-4R alpha expression on macrophages / neutrophils (in LysM(cre)IL-4R alpha(-lox) animals) had minimal effect on the outcome of L. mexicana infection compared with control (IL-4R alpha(-/flox)) mice. In contrast, CD4(+) T cell specific (Lys(cre)IL-4R alpha(-lox)) IL-4R alpha(-/-) mice infected with L. mexicana developed small lesions, which subsequently healed in female mice, but persisted in adult male mice. While a strong Th1 response was manifest in both male and female CD4(+) T cell specific IL-4R alpha(-/-) mice infected with L. mexicana, induction of IL-4 was manifest in males but not females, independently of CD4(+) T cell IL-4 responsiveness. Similar results were obtained using pan-T cell specific (iLck(cre)IL-4R alpha(-/lox)) IL-4R alpha(-/-) mice. Collectively these data demonstrate that upon infection with L. mexicana, initial lesion growth in BALB/c mice is dependent on non-T cell population(s) responsive to IL-4/IL-13 while progressive infection is dependent on CD4(+) T cells responsive to IL-4.