Picture of a black hole

Strathclyde Open Access research that creates ripples...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of research papers by University of Strathclyde researchers, including by Strathclyde physicists involved in observing gravitational waves and black hole mergers as part of the Laser Interferometer Gravitational-Wave Observatory (LIGO) - but also other internationally significant research from the Department of Physics. Discover why Strathclyde's physics research is making ripples...

Strathprints also exposes world leading research from the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

Discover more...

BALB/c mice deficient in CD4(+) T cell IL-4R alpha expression control Leishmania mexicana load although female but not male mice develop a healer phenotype

Bryson, Karen and Millington, Owain and Mokgethi, Thabang and McGachy, Adrienne and Brombacher, Frank and Alexander, James (2011) BALB/c mice deficient in CD4(+) T cell IL-4R alpha expression control Leishmania mexicana load although female but not male mice develop a healer phenotype. PLOS Neglected Tropical Diseases, 5 (1). pp. 1-10. ISSN 1935-2727

[img]
Preview
PDF (Bryson Millington Mokgethietal PLoS 2011)
BrysonMillingtonMokgethietalPLoS2011.pdf - Final Published Version
License: Creative Commons Attribution 4.0 logo

Download (823kB) | Preview

Abstract

Immunologically intact BALB/c mice infected with Leishmania mexicana develop non-healing progressively growing lesions associated with a biased Th2 response while similarly infected IL-4R alpha-deficient mice fail to develop lesions and develop a robust Th1 response. In order to determine the functional target(s) for IL-4/IL-13 inducing non-healing disease, the course of L. mexicana infection was monitored in mice lacking IL-4R alpha expression in specific cellular compartments. A deficiency of IL-4R alpha expression on macrophages / neutrophils (in LysM(cre)IL-4R alpha(-lox) animals) had minimal effect on the outcome of L. mexicana infection compared with control (IL-4R alpha(-/flox)) mice. In contrast, CD4(+) T cell specific (Lys(cre)IL-4R alpha(-lox)) IL-4R alpha(-/-) mice infected with L. mexicana developed small lesions, which subsequently healed in female mice, but persisted in adult male mice. While a strong Th1 response was manifest in both male and female CD4(+) T cell specific IL-4R alpha(-/-) mice infected with L. mexicana, induction of IL-4 was manifest in males but not females, independently of CD4(+) T cell IL-4 responsiveness. Similar results were obtained using pan-T cell specific (iLck(cre)IL-4R alpha(-/lox)) IL-4R alpha(-/-) mice. Collectively these data demonstrate that upon infection with L. mexicana, initial lesion growth in BALB/c mice is dependent on non-T cell population(s) responsive to IL-4/IL-13 while progressive infection is dependent on CD4(+) T cells responsive to IL-4.