Ulijn, R.V. and Baragana, B. and Halling, P.J. and Flitsch, S.L. (2002) Protease-catalyzed peptide synthesis on solid support. Journal of the American Chemical Society, 124 (37). pp. 10988-10989. ISSN 0002-7863
Full text not available in this repository. (Request a copy from the Strathclyde author)Official URL: http://dx.doi.org/10.1021/ja026912d
Abstract
The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer.
| Item type: | Article |
|---|---|
| ID code: | 318 |
| Keywords: | Soluble polymeric support, safety catch linker, enzymatic- synthesis, organic-solvents, biocatalysis, enzymes, phase, chemistry, resin, Chemistry |
| Subjects: | Science > Chemistry |
| Department: | Faculty of Science > Pure and Applied Chemistry |
| Related URLs: | |
| Depositing user: | Users 16 not found. |
| Date Deposited: | 10 Mar 2006 |
| Last modified: | 15 Jun 2012 13:50 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/318 |
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