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Protease-catalyzed peptide synthesis on solid support

Ulijn, R.V. and Baragana, B. and Halling, P.J. and Flitsch, S.L. (2002) Protease-catalyzed peptide synthesis on solid support. Journal of the American Chemical Society, 124 (37). pp. 10988-10989. ISSN 0002-7863

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Abstract

The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer.

Item type: Article
ID code: 318
Keywords: Soluble polymeric support, safety catch linker, enzymatic- synthesis, organic-solvents, biocatalysis, enzymes, phase, chemistry, resin, Chemistry, Biochemistry, Colloid and Surface Chemistry, Chemistry(all), Catalysis
Subjects: Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
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Depositing user: Users 16 not found.
Date Deposited: 10 Mar 2006
Last modified: 04 Sep 2014 11:25
URI: http://strathprints.strath.ac.uk/id/eprint/318

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