Picture of person typing on laptop with programming code visible on the laptop screen

World class computing and information science research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

Explore

Evaluation of nerve cell toxicity in vitro by electrophysiological and biochemical methods

Xie, K and Harvey, A L (1993) Evaluation of nerve cell toxicity in vitro by electrophysiological and biochemical methods. Toxicology in Vitro, 7 (3). pp. 275-279. ISSN 0887-2333

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

The acute toxicity of the first nine standard chemicals in the Multicentre Evaluation of In Vitro Cytotoxicity Tests (MEIC) programme was evaluated in the NG108-15 neuroblastoma cell line by monitoring changes in cell resting membrane potential (RMP) and by using the MTT assay to measure cell viability. Cells were differentiated with dibutyryl-cAMP and then exposed to different concentrations of the chemicals for 1 hr or 24 hr. At each concentration and time point, RMPs were measured from about 30 differentiated cells and MTT assays were performed on parallel cultures. IC(50) values were obtained from linear regression analysis. The results showed that the IC(50)s from MTT assays correlated closely with those from RMP measurements (r = 0.983 for 1 hr exposure; r = 0.933 for 24 hr exposure). IC(50)s of amitriptyline and diazepam were 0.1-1.9 mm; alcoholic compounds (isopropanol, ethylene glycol, ethanol and methanol) had IC(50)s from 121.5 mm to 3731.9 mm; paracetamol, aspirin and ferrous sulphate had intermediate cytotoxicity (IC(50) 2.6-53.5 mm). IC(50)s decreased markedly with increased exposure time. RMP is expected to be a sensitive indicator of the health of nerve cells; however, its measurement in a large number of cells is laborious. MTT assays are rapid, and the close correlation between IC(50)s in the two types of assay suggests that MTT assays could be used to evaluate cytotoxicity in neuronal cells in vitro.