Harvey, Alan L and Kornisiuk, Edgar and Bradley, Karen N and Cerveñansky, Carlos and Durán, Rosario and Adrover, Martin and Sánchez, Gonzalo and Jerusalinsky, Diana (2002) Effects of muscarinic toxins MT1 and MT2 from green mamba on different muscarinic cholinoceptors. Neurochemical Research, 27 (11). pp. 1543-1554. ISSN 0364-3190Full text not available in this repository. (Request a copy from the Strathclyde author)
MT1 and MT2, polypeptides from green mamba venom, known to bind to muscarinic cholinoceptors, behave like muscarinic agonists in an inhibitory avoidance task in rats. We have further characterised their functional effects using different preparations. MT1 and MT2 behaved like relatively selective muscarinic M1 receptor agonists in rabbit vas deferens, but their effects were not reversed by washing or prevented by muscarinic antagonists, although allosteric modulators altered responses to MT1. Radioligand binding experiments indicated that both toxins irreversibly inhibited [3H]N-methylscopolamine binding to cloned muscarinic M1 and M4 receptors, and reduced binding to M5 subtype with lower affinity, while they reversibly inhibited the binding of [3H]prazosin to rat cerebral cortex and vas deferens, with 20 fold lower affinity. High concentrations of MT1 reversibly blocked responses of vas deferens to noradrenaline. MT1 and MT2 appear to irreversibly activate muscarinic M1 receptors at a site distinct from the classical one, and to have affinity for some alpha-adrenoceptors.
|Keywords:||animals, molecular cloning, elapidae, male, peptides, protein binding, rabbits, radioligand assay, rats, receptors adrenergic alpha-1, receptors muscarinic, wistar, Pharmacy and materia medica, Cellular and Molecular Neuroscience, Biochemistry|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Pure Administrator|
|Date Deposited:||13 Jul 2011 08:56|
|Last modified:||04 May 2016 18:48|